十三种淡水养殖鱼类的DNA含量

DNA CONTENT FROM THIRTEEN SPECIES OF CULTURED FRESHWATER FISHES IN CHINA

通过流式细胞仪测定了青鱼等十三种我国主要淡水养殖鱼类外周血红细胞的ＤＮＡ含量。其中青鱼、草鱼、鲢和鳙的ＤＮＡ含量比较接近，其２Ｃ值分别为２．２０、２．１８、２．１８、２．１５ｐｇ，四种鱼相比较，差异不显著。兴国红鲤、镜鲤、荷包红鲤的ＤＮＡ含量也比较接近，其２Ｃ值分别为３．８０、３．７３、３．７６ｐｇ。相比较差异也不显著。银鲫的ＤＮＡ含量为６．０９ｐｇ／Ｎ，与鲤比接近３：２。尼罗罗非鱼和奥利亚罗非鱼的ＤＮＡ含量几乎相等，分别为２．２７、２．２０ｐｇ／Ｎ。虹鳟、鲟和团头鲂的ＤＮＡ含量分别为３．４７、１１．７３、２．６６Ｐｇ／Ｎ。由ＤＮＡ含量可见这些鱼类的血缘关系。最后讨论了测定鱼类体细胞ＤＮＡ含量的方法等问题。

Flow cytometric DNA analysis of peripheral blood cells was used to determine the DNA contents from thirteen species of cultured freshwater fishes in China.The DNA contents of black carp (Mylopharyngodon piceus),glass carp(Ctenopharyngodon idellus),silver carp(Hypophthalmichthys molitrix) and bighead carp(Aristichthys nobilis) were 2.20,2.18,2.18 and 2.15pg/N respectively,which showed no significant difference between each two of them.Three common carps,Xinguo red carp(Cyprinus carpio var singuonesis),C.carpio with mirror carp and C.carpio with red colour,almost had the same content, 3.80,3.73 and 3.76pg/N respectively and no significant difference was observed.Two tilapia (Sarotherodon niloticus and S.aurea) also had the similar DNA content of 2.27pg/N and 2.22pg/N.The DNA contents from rainbow trout(Oncorhynchus mykiss),amur sturgeon(Acipenser schrensch) and blunt snout bream(Megalobrama amblycephala )were 3.47,11.73 and 2.66pg/N.respectively. Finally,the determination of systematic relationship of fish species from the DNA contents was suggested.