四种吸虫成虫抗原的酶联免疫印迹分析

Analysis of Antigens of Four Adult Trematodes by ELIB

应用酶联免疫印迹分析姜片吸虫、肝片形吸虫、华支睾吸虫和日本血吸虫成虫抗原,结果显示,各种抗原的蛋白多肽均多达数十个成分,四种吸虫抗原与同源兔免疫血清产生颜色较深、数量较多(20～40条)的免疫反应区带,与其他三种异源免疫血清及日本血吸虫感染血清显示数量不等的交叉反应性区带。四种抗原共有10个特异的抗原性多肽。

ELIB was used for the analysis of soluble adult antigens of Fasciolopsis buski, Fasciola hepatica, Clonorchis sinensis and Schistosoma japonicum (S.j. ) .The results revealed:1.Each antigen consisted of about 35~57 polypeptides in which mappings there were obvious differences.2.In ELIB, the bands ( antigenic polypeptides ) were seen in relatively large number and heavily stained with homologous rabbit immune sera, various number of cross-reactive bands with heterologous rabbit immune sera or S.j. infected rabbit sera were shown also. In these four antigens, at least ten specific bands were identified.