熊猴实验感染人乙肝病毒的动态病理学研究

A Stuty of Developmental Pathology of Experimental Infection by Human Hepatitis B Virus(HHBV) in Macaca Assamensis

本项研究选7只无自然感染HHBV的健康熊猴,实验感染HHBV。在这批熊猴出现血清学阳性的证据后,对其做肝活检(分早、中、晚期),经光、电镜动态病理学观察,并结合组化、免疫组化和分子原位杂交等法定位检测肝细胞内HHBV标志物。结果各猴肝脏组织病理学和超微病理学的动态变化与乙肝病人相似;并在猴肝细胞内定位检测出HBsAg、HBcAg和HBV-DNA复制,建立了理想的乙肝模型。

Seven normal Macaca assamensis were experimentally infected by HHBV. We performed the biopsy of liver to Macaca assamensis after the evidence for positive reaction of the serum had been given.The changes of developmental pathology of their livers were observed by light and electric microscopy and HHBV in the hepatocyte were locatively examined by histochemistry, immunohistoche-mistry and in site hybridization. The results showed that the pathologically and ultrapathologically developmental changes in the liver of every experimental animals is similah to that of the patients with hepatitis B. It was locatively examined that HBsAg,HBcAg and HBV-DNA were all positive in liver cells. So that an ideal pattern of hepatitis B was set up .