蛋白质能量营养不良小鼠腹腔巨噬细胞膜结构及吞噬功能的研究

A Study on the Cytomembrane Structure and Phagocytosis of the Peritoneal Macrophages(M ) ofMice with PEM

喂小鼠去蛋白饲料造成小鼠蛋白质能量营养不良（ＰＥＭ），用扫描电镜（ＳＥＭ）和体外鸡红细胞吞噬试验观察其腹腔巨噬细胞（Ｍ）膜表面结构及吞噬功能变化。结果，随喂去蛋白饲料时间延长，小鼠体重下降，血清蛋白含量降低，Ｍ膜表面绒毛样凸起基本消失，Ｍ 的吞噬功能亦下降；改喂含１７％蛋白质的饲料后，其腹腔Ｍ 膜表面结构和吞噬功能均可逐步恢复正常．提示，蛋白质缺乏造成的ＰＥＭ直接导致了Ｍ 膜结构损伤和吞噬功能下降，但如及时补充蛋白质，这种损伤是可逆的．

n this study, protein--energy malnutrition(PEM) of mice was resulted in by feeding protein- free blood, then normal diet (containing 17% protein ) for these with PEM. We observedcytomembrane structure and phagocytosis of peritoneal M of the mice with PEM by means ofscanning electron microscopy(SEM) and phagocytosis-- test in vitro. The resultS showed: 1. The viili--like Protrusion on the surface of M membrane were reduced progressively up to nearly disappearalong with the weight reducing of mice with PEM. 2. The phagocytic precentage and index ofperitoneal M in mice with PEM were significantly lower than that in normal mice. 3. It could berecovered through protein supplementation in thee.