PWM激活的淋巴细胞亚群的功能研究

The Study of Function in PWM-induced Lymphocytes Subsets

用放射性标记化合物掺入法研讨ＰＷＭ诱导人血淋巴细胞亚群的功能及其调节。实验表明，ＰＷＭ细胞对ＬＰＳ活的Ｂ细胞转化有促进作用，受４０Ｇｙ辐照后，它的作用显著下降，但仍有一定加强效应。ＰＷＭ细胞对ＰＷＭ激活的淋巴细胞转化的促进作用不明显，对ＣｏｎＡ和ＰＨＡ激活的细胞无效。用玫瑰花环法分离Ｔ、Ｂ细胞，用ＣＤ４、ＣＤ３和Ｂ３株单克降抗体（ＭｃＡｂ），以亲和层析和Ｐａｎｎｉｎｇ法分离各淋巴细胞亚群，分别研究ＰＷＭ对各亚群的激活及相互调节作用．结果表明：Ｂ细胞被ＰＷＭ激活强于Ｔ细胞，ＰＷＭ对Ｂ和非ＣＤ４细胞激活作用相当，ＣＤ２细胞次之，ＣＤ４细胞最弱．Ｔ细胞或ＣＤ４细胞与Ｂ细胞均有协同作用，前者作用更大．ＰＷＭ－非ＣＤ４和ＰＷＭ—Ｂ细胞无协同作用，ＣＤ６细胞与Ｂ细胞有抑制作用。

he function and regulation in PWM-induced human blood lymphocyte subsets were studied by incorporation method of radioisotope labelling This study found the enhanced effect of PWM- induced cells on human blood B lymphocytes stimulated by LPS After exposed to 40Gy the enhancedeffect decreased significantly ,but it still had certain effect on B lymphocyets,for the effect Of PWM-induced cells on lymphosytes stimulated by PWM there is no evidence,and the effect of PWM- induced cells on lymphocytes on lymphocytes stimuated by Con A and PHA did not exist.T and B lymphocytes were separated by rosetting procedures and three lmphocytes were separated subsets were separated by CD4, and B monoclonal antibodies ,The effect of stimulation and regulation oflymphocyte subsets wal studied respectively ,This paper reflected that PWM can stimulate both T and B cells but B cells were more sensitive to the same as thatOf non-CD4 cells. The response of CD3 Cells tO PWM was lower,and the CD4 cells was the lowest.Both T and CD4 lymphocytes were synergistic to B lymphocytes but such a kind of synergistic effect in T cells more obvious than that in CD4 group The suppressive effect of CD8 cells on B cells was found .