IL—4、γIFN及TNF对人骨髓瘤细胞系（U266、XG—7）与血管内皮细胞粘附效应的影响

Effect of IL-4, rIFN and TNF on the Adhesive interaction Human Myeloma Ceu (U266/XG-7) and the Vascular Cells

５１Ｃｒ标记多发性骨髓瘤细胞系的方法，分析它们与血管内皮细胞间的粘附效应和细胞团子（ＩＬ－４、ｒＩＦＮ、ＴＮＦ）对该粘附的调控作用，并探讨这３种因子对正常血管内皮细胞粘附分子表达及可溶性因子分泌的影响．结果表明：（１）ＩＬ－４、ｒＩＦＮ、ＴＮＦ之间的联合应用可显著提高Ｕ２６６ＸＧ—７与血管内皮细胞的粘附能力．（２）ＩＬ—４、ｒＩＦＮ、ＴＮＦ可提高血管内皮细胞表面ＣＤ５４、ＣＤ４４的表达，并不同程度地促进了内皮细胞分泌ＩＬ—６、Ｇ—ＣＳＦ和ＴＮＦ等因子。提示这３种细胞因子可能有助于多发性骨髓瘤细胞的转移扩散．

y labelled the myeloma cells with 51Cr,we analysed the adhesive interaction between muelomaCell lines and the endothelial cells and the cytokines (I1-4,rIFN,TNF) regulation effeft on them .Morever ,the effect of these three cytokines on the adhesion molecules espression and cytokine secretion Of normal endothelial cells was also studyed the results indicated:1 Combination of IL- 4,rIFN and TNF can markedly improve the adhesive interaction beteewn U266/XG-7 and the endothetal cells 2.Cytokines (IL-4, rIFN,TNF) Can induce the increase in the expression of CD54, CD44 On theendothelial cells. All the results Suggested that these three cytokines-IL-4 , rIFN and TNF maycontribute to the metastasis of myeloma cells.