应用聚合酶链反应诊断肺炎支原体感染

DIAGNOSTIC EFFET OF PCR ON INFECTION OF MYCOPLASMA PNEUMONIAE

肺炎支原体（ＭＰ）是呼吸道感染的常见病原。我们应用聚合酶链反应（ＰＣＲ）技术检测Ｍｐ一ＤＮＡ，该方法具有早期，快速、敏感，特异性高等特点。７５例疑ＭＰ感染患儿的咽分泌物标本，阳性３０例（４０％）。ＭＰ感染大多数引起鼻咽炎、支气管炎、不典型肺炎。通过分析发病年龄，我们发现５岁～１１岁年龄组感染率最高，１岁～３岁组次之，最小患病年龄为４个月，表明ＭＰ感染向低龄儿方向发展。ＰＣＲ可以早期、快速对ＭＰ感染做出诊断，对我们研究ＭＰ感染帮助很大，可以使我们重新认识ＭＰ的发病率。

Mycoplasma pneumoniae is a common pathogenic germ of respiratory infections. Wehave utilized polymerase chain reaction(PCR )technique for revealing Mycoplasma Pneumoniae一DNA,which has been proved to be quick,sensitive ,specific and effective in the early stage of diagnose. OF 75suspectable Mycoplasma Pneumoniae(MP)infection children whose pharyngnal excretion have been in-spected with PCR,30 children are positive(40 per cent).We notice that the majority of MP infectionpatients represent mostly the symptoms of rhinitis,pharyngitis,bronchitis and pneumonia. Some patientsare complicated by nonpulmonary focus. With analysing the relationship between age and infection rateof MP,we find that the infection rate in 5一11 year group is higher than l一3year group,with theyoungest patient is a four months child. It demonstrates that the MP infection lias inclined to baby. PCRhas afforded a early and quick diagnostic method for MP infection,which will play a great role in MP in-fection research and bring about reevaluating the incidence of MP infection.