反相高效液相色谱法快速测定人唾液中甲硝唑及其代谢产物

REVERSED─PHASE HPLC DETERMINATION OF METRONIDAZOLE AND ITS METABOLITE IN HUMAN SALIVA

应用ＬＣ一６Ａ型高效液相色谱仪，建立了快速测定唾液中甲硝唑及其代谢产物的方法。固定相为Ｓｈｉｍ－ｐａｃｋ ＣＬＣ－ＯＤＳ柱，甲醇－水（２７：７３）作流动相，流速１．２ｍｌ／ｍｉｎ，检测波长３ｌ８ｎｍ。甲硝唑平均回收率为９９．４０％，日内和日间变异系数分别为３．７２％和３．９８％。最低检出浓度为０．０２５μｇ／ｍｌ，线性范围０．０５～２０μｇ／ｍｌ（ｒ＝０．９９９９）。对唾液与血清中甲硝唑浓度的相关性作了初步探讨。

A reversed一phase HPLC method has been developed for rapid determination ofmetronidazole and its metabolite in human saliva by using a Model LC一6A HPLC with two pumps anda Shim一pack CLC一ODS colum. The mobile phase,mixture of methanol and redistilled water(27 :73,byvol),was used at a flow rate of l.2ml/min. The samples was monitored at 3l 8 nm wavelength. Themean recoverv of metronidazole in saliva was 99.40%,Within一day and between一dav variations were3.72%and 3.98%,respectively. The measurable lowest level was 0.025 μg/ml and the standard curve ofmetronidazole was linear over the concentration range of 0.05～20 μg/ml(r=0.9999 ).The pharma-coknetics of metronidazole in human after oral administration and the relativity between saliva andserum concentrations were studied.