一种新型的生物人工肝—微囊肝细胞

A NEW BIOARTIFICIAL LIVER-ENCAPSULATEDHEPATOCYTES

采用海藻酸盐—聚赖氨酸—海藻酸盐膜成功地将大鼠游离肝细胞制备成微囊肝细胞。短期体外培养显示微囊肝细胞的尿素和白蛋白合成功能与游离肝细胞相仿。组织学检查显示植入大鼠腹腔内的微囊肝细胞35天后仍有半数存活。将微囊肝细胞植入氨基半乳糖引起肝衰竭的大鼠腹腔内可显著地提高其存活率,初步研究的结果表明微囊肝细胞可能有治疗肝衰竭的临床应用价值。

Us ing alginate-polylysine-alginate membrane, we have successfully en- capsulated rat hepatocytes with little loss of viability. Urea and albumin release from encapsulated hepatocytes were comparable to that from non-en- capsulated hepatocytes during short-term culture. Histological studies also showed that more than 50% of encapsulated hepatocytes remained viable 35 days after implantation in peritoneal cavity of rats. Preliminary studies also showed significant increase in survival rate after encapsulated hepato- (Continued on page 257 ) (Continued from page 235 ) cytes being transplanted into galactosamine-induced FHF rats. In conclu- sion, transplantation of microencapsulated hepatocytes provides a potential clinical treatment for hepatic failure. Anthony Mien-Fang Sun, Honorary Professor of Shanghai Medical University. Professor of Phsiology, University of Toronto, Senior Research Scientist, Connaught Research Institute, Toronto, Canada. Shanghai Biological Engineering Centre, Academia Sinica,