卵巢癌患者机体免疫状况的研究及意义

Study on Immunologie Status of Patients with Ovarian Cancer

应用单克隆抗体免疫荧光技术检测48例卵巢癌患者的外周血淋巴细胞亚群,发现早期患者即表现CD_1、CD_2、CD_(20)及CD_4/CD_8比值明显下降,CD_8明显增高,当病变进一步发展时,这些改变愈加明显。手术和化疗对患者的免疫系统有一定的影响。同时,应用淋巴细胞增殖和增殖抑制试验检测34例患者的免疫抑制因子,发现术前卵巢癌患者血清可明显抑制PHA诱导的淋巴细胞增殖反应,且这种抑制呈剂量依赖关系,即使稀释到1:4000时仍有明显抑制活性。

The peripheral blood lymphocyte(PBL) suhsets m 18 patients with ovarian carcmoma were in-vestigated by using anti-human lymphocyte monocioiial armbody and indirect imnumofhiorescenct technique. The results showed that .during the early stage of the rancer ,CD3 ,CD4 .CD20 and CD4/ CD8 ratio were significantly decreased.but CD8 was markedly increased ,wtth the dtsease progre.ss ing,the immunologieal changes were more and more evident. Operation and chemotherapy had an effect on the patient' s immunocompetence- The suppressive activity of immunosuppressive factors (ISF) in serum from ovarian cancer patients was observccl by a mod'fied lymphocvte prohferation inhihition test. In pre-operational patients with ovarian cancer. suppressive effect of serum on PHA-induced lymphocyte proliferalion was significantly greater tlian that from cancer-removed pa tients. The suppression was dose-dependent ,but when the serum was diluted to 1 :4000,the suppressive activity was still observed.