均匀设计在长春花丛生芽培养中的应用

METHOD OF UNIFORM DESIGN AND ITS APPLICATION TO SHOOT CULTURE OF CATHARANTHUS ROSEUS

均匀设计方法从试验点在试验范围内＂均匀分散＂出发，可提高单次实验的效率，以较精细的多元统计分析试验结果，提高了分析结果的可靠性．在不降低试验精度的情况下，大大减少试验次数，降低成本．本文将此方法用于长春花丛生芽培养过程分化条件的优选工作．结果表明，此方法对于多因素多水平的试验设计有明显的优点．

he method of uniform design is introduced in this paper.By uniform design,the effects of different combinations of 6-BA with NAA or 2,4-D on the formation of multiple shoots of Catharanthus roseus(C.roseus) are studied. By analyzing regression equations from the experimental data.we found out clearly that Murashige-Shoog(MS) medium supplemented with 7.0 mg/L 6-BA and 1.0 mg/L NAA strongly stimulates the formation of multiple shoots of C.roseus, while the medium free of 2,4-D is beneficial to the differentiation of C.roseus.