胃幽门螺杆菌抗体定量测定及对比观察

ELISA for Serodiagnosis of Helicobacter Pylori in Gastroduodenal Disease as Compared with Other Techniques

以ELISA(酶联免疫吸附法)幽门螺杆菌抗体定量法测定了187例慢性胃病患者(均经培养和/或病理确诊)的血清特异性幽门螺杆菌(HP)IgG抗体。检出率为75.4％,敏感性为93.8％,特异性为100％。该法的阳性检出率高于培养法、病理HE染色法和尿素酶试验,有显著差异(P<0.05),而再三者间则无显著差异(P>0.05)。结果提示ELISA-HP抗体定量法具有检出率高、敏感、特异、可靠、经济、简便的优点,具可避免或减少胃镜检查的痛若,临床值得推广应用。

Thirteen isolates of helicobagter pylori were pooled as antigen to detect the specific IgG antibody to H. pylori in 187 patients with various gastroduodenal diseases. These Patients were diag-nosd by way of gastroendoscopic examination. The sensitivity of ELISA was 93. 08% and specificity 100%. The antibody to H. pylori had no cross-reactivity with the extracts of C. jujuni,C. fetus and E. coli. Three to five biopsy specimens were obtained in each of 187 patients. The results indicated that the detection rate of ELISA was 75. 4%. It was higher than that of the culture,urease test and H and E stain which were 60. 03%, 56. 7%and51- 33% respectively. ELISA detection rate 75. 4% was the highest in all methods and the rate differences (P<0. 025) were statistically significant. It indicated that such a serodiagnosis assay of gastric infection disease was of high sensitivity, specificity and reliability. It may also be used to screen patients prior to endoscopy and follow-up the patients with gastroduodenal ulcer during treatment.