抗莱氏支原体单克隆抗体的研制

A Study on Monoclonal Antibodies against A.Laidlawii Mycoplasma

通过细胞融合技术，筛选出ＭＡＬ－１和ＭＡＬ－２两株抗莱氏支原体（Ａ．Ｌａｉｄｌａｗｉｉ，Ａ．Ｌ）单克隆抗体（单抗）。通过ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ印迹法测得单抗相对应的抗原分子量均为６７．４６ｋｕ。用间接免疫荧光法（ＩＦＡ）检测，单抗与细胞培养中另外３种常见污染的支原体和呼吸道肺炎支原体无交叉反应。将单抗用于检测支原体污染的细胞培养物，结果表明，此单抗能特异性地检出莱氏支原体。故可用其推断支原体污染途径，便于制定防治措施。

By using cell fusion technique,two monoclonal antibodies,MAL-1 and MAL-2,against A.Laidlawii(A.L) were produced.The molecular weight of the A.L antigens was 67.46ku as determined by SDS-PAGE and Western blot.These two MAbs showed no cross-reaction to other four Mycoplasmas,M.Hyorhinis,M.Orale,M.Ariginini and M.Pneumoniae,as tested by indirect fluorescence assay(IFA).Our study showed that these antibodies could be used for specific detection of A.Laidlawii.