平菇杂交育种的研究

Researches on Cross-breeding ot Pleurotus

本文报道采用杂交方法进行平菇育种的研究,通过单核菌丝的特性观察,配对杂交,拮抗试验,同工酶分析,出菇试验和品比试验,筛选出了2个高温型的高产杂交组合13—8×8—54和26—11×8—54。在品比试验中,13—8×8—54的生物转化率为107.2％,单产比优势亲本和对照增产22.6％;26—11×8—54单的生物转化率为103.2％,单产比优势亲本和时照分别增产24.0％和17.7％。研究结果表明采用不同遗传类型的亲本进行杂交,这是选育平菇新品种的一条快速、有效的途径。

In this paper, the cross-breeding of Pleurotus were studied, through the survey of the characteristics of simple-karyon mycelium. paired cross-breeding, antagonism and strain test, isozymes analysis and flushing fruitbody, 13-8×8-54 and 26-11×8-54 that are the high-temperature resistant and high-yielding heterokayons were screened,In the strain test, the biological transformation of 13-8×8-54 and 26-11×8-54 was 107.2% and 103.2% respectively, perunit yield of the former was/22.6% higher than that of the high-yielding parent and the contrast, and that of the latter increased by 24.0% and 17.7% as compared with that of the hig h-yielding parent and the contrast respectively. The result showed that crossing the parents by different genetic types was a first and effective way to breed new strains of Pleurotus.