DDT、DDE对生物膜表层流动性及荧光参数的影响

Effects of DDT, DDE on Membrane Fluidity

以ＡＮＳ（８－Ａｎｉｌｉｎｏｎａｐｈｔｈａｌｅｎｅ－１－Ｓｕｌｐｈｏｎｉｃａｃｉｄ）为荧光探剂，观察了对，对’－滴滴涕（Ｐ，Ｐ’－ＤＤＴ）、对，对’－敌敌伊（Ｐ，Ｐ’－ＤＤＥ）对大鼠脑突触体膜、肝线粒体膜和肝微粒体膜表层流动性及荧光参数的影响。结果显示：较高浓度的ＤＤＴ或ＤＤＥ能使脑突触体膜ＡＮＳ的荧光偏振度降低，而对肝微粒体膜和肝线粒体膜的荧光偏振度无明显影响。ＤＤＴ、ＤＤＥ增强ＡＮＳ－生物膜复合物的荧光强度，此种效应的产生不是由于改变了膜上ＡＮＳ周围微环境的极性，而是ＡＮＳ分子在生物膜上的结合数量增加所致。

The effects of DDT and DDE on membrane fluidity of the brain synaptosome, hepatic microsome and mitochondria were investigated in rat by using ANS fluorescence polarization technique. The results showed that DDT and DDE were able to lower the value of polarization of rat synaptosome at the high concentrations, and DDT and DDE did not showed any effects on the polarization of microsome or mitochondria of rat liver, DDT and DDE enhanced the fluorescence intensity of the ANS-membrane complex, suggesting that the effect is not due to the increase in the quantum yield of ANS but an increase in the amount of ANS bound to the membrane.