脉络通和青黛治疗家兔肾小球肾炎的实验研究

Experimental Studies on Immune-complex In Situ Type Glumerulonephritis in Rabbits Treated with Mai-Luo-Tong and Natural Indigo

给兔注射阳离子化牛血清白蛋白复制免疫复合物肾炎后，分别给中药脉络通和青黛治疗。结果表明：治疗后，蛋白尿在脉络通治疗组（Ｍ）、青黛治疗组（Ｑ）明显减少，尽管Ｑ组与实验对照组（Ｃ）相比无统计学差异（Ｐ＞０．０５），但Ｍ组与Ｃ组相比Ｐ＜０．０５。在荧光显微镜下，见ＩｇＧ和Ｃ３呈颗粒状和线样沉积在肾脏毛细血管壁，Ｍ组ＩｇＧ、Ｑ组Ｃ３沉积的强度均较Ｃ组弱（Ｐ均＜０．０５）。光镜和电镜下３组均显示肾小球基底膜不规则增厚，上皮下电子致密物沉积，但在肾小球毛细血管内未见微血栓、红细胞及血小板积聚和白细胞嵌顿。其系膜细胞数，在Ｍ、Ｑ、Ｃ组中分别为６６．５５±７．７５、９２．８７±１７．８９、９９．４０±１８．５３个，Ｍ、Ｑ组与Ｃ组相比有统计学差异（Ｐ＜０．０１）。治疗兔肾小球亦无纤维化。

By injection of C-BSA,immune-Complex in situ type glomerulonephritis was reproduced in rabbits and treated with Mai-Luo-Tong(group M)and Natural Indigo(group Q).The results showed that proteinuria in the treated groups M and Q was decreased.The difference between group M and control group was statistcally significant(P<0.05).IgG and C3 deposited on renal capillary wall as revealed by fluorescence microscopy.Deposition of IgG in group M and C3 in group Q were milder than in C group,Under light and electron microscope,although in all groups glomerular basement membrane was irregularly thickened and dense eletron deposited under epithelium in the treated group,microthrombus,erythrocytes and platelets aggregation and leukocytes impactionn were not seen within glomerular capillaries.Also in groups M,Q,C mesangium cell count was 66.55±7.75,92.87±17.89 and 99.40±18.53 respectively, and significant differences were found between groups M and Q and groups C(P<0.01).No apparrent glomerular fibrosis was found in the treated groups.