人口腔粘膜鳞癌中Ha－ras癌基因第12位密码子G→T突变的研究

Detection of G→T Mutation at Codon 12 of the C-Ha-ras Oncogene in Human Oral Mucosal Squamous Cell Carcinoma

采用聚合酶链反应和寡核苷酸探针杂交技术，检测２１例口腔粘膜鳞癌组织中Ｈａ－ｒａｓ癌基因第１２位密码子Ｇ→Ｔ突变，结果有６例存在这种点突变（２８．６％），而１０例正常口腔粘膜组织无１例发生这种突变。提示：Ｈａ－ｒａｓ癌基因第１２位密码子Ｇ→Ｔ突变可能导致口腔癌中癌基因的激活。并对点突变的临床意义进行了讨论。

The polymeras chain reaction(PCR)was used to amplify the DNA sequence of Ha-ras oncogene in the tissuses of oral mucosal squamous cell carcinoma and the G→T mutation at codon 12 of the C-Ha-ras oncogene was detected by hybridization with mutation specific oligonucleotide probes.The results showed that the G→T mutation rate was 28.6%(6/21)In human oral cancers,but no mutation was found in 10 normal oral mucosal tissues.This study suggested that the G→T mutation at codon 12 of the C-Ha-ras oncogene may lead to activation of C-Ha-ras oncogene.The clinical significance of the mutation was also discussed.