氧化修饰LDL及VLDL对兔主动脉平滑肌细胞单核细胞趋化蛋白1mRNA表达的作用

Effects of Oxidatively Modified Low Density Lipoprotein and Very Low Density Lipoprotein on the Expression of Monocyte Chemotactic Protein 1 mRNA in Rabbit Aortic Smooth Cells

单核细胞趋化蛋白１（ＭＣＰ－１）是一种强的单核细胞趋化因子，在体外可由多种细胞分泌，包括血管平滑肌细胞（ＳＭＣ）。本研究采用斑点杂交方法检测了氧化修饰低密度脂蛋白（ＯＸ－ＬＤＬ）、极低密度脂蛋白（ＶＬＤＬ）及氧化修饰极低密度脂蛋白（ＯＸ－ＶＬＤＬ）对培养的兔主动脉ＳＭＣＭＣＰ－１ｍＲＮＡ表达的作用。结果表明，培养的ＳＭＣ可表达ＭＣＰ－１ｍＲＮＡ，而且ＳＭＣ暴露于ＯＸ－ＬＤＬ、ＶＬＤＬ及ＯＸ－ＶＬＤＬ后，其ＭＣＰ－１ｍＲＮＡ表达水平明显增高，分别增高约１１倍、６倍和２０倍。因此我们认为ＯＸ－ＬＤＬ、ＶＬＤＬ及ＯＸ－ＶＬＤＬ可以刺激培养的兔主动脉ＳＭＣＭＣＰ－１ｍＲＮＡ的表达。

The migration of monocytes into subendothelial space is one of the earliest events in the pathogenesis of atherosclerosis.Monocyte chemotactic protein 1(MCP-1)is a potent monocyte chemo-attractant secreted by many cells in vitro,including vascular smooth muscle cells.In this study we have examined the effects of oxidatively modified LDL(OX-LDL),very low density lipoprotein(VLDL)and oxidativelv modified VLDL(OX-VLDL)on the expression of MCP-1 mRNA in cultured rabbit aortic smooth muscle cells(SMCs)using dot blotting analysis.The results showed that cultured SMCs could express MCP-1 mRNA and the levels of MCP-1 mRNA were increased significantly in SMCs when exposed to OX-LDL(11-fold),VLDL(6-fold)and OX-VLDL(20-fold).We conclude that OX-LDL,VLDL and OX-VLDL can stimulate the expression of MCP-1 mRNA in cultured rabbit aortic SMCs.