急性白血病患儿血清可溶性白细胞介素2受体水平的研究

Study of Serum Soluble Interleukin-2 Receptor Levels in Children with Acute Leukemia

采用双抗体夹心酶联免疫试验法（ＥＬＩＳＡ）对６５例急性白血病患儿血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）水平进行检测。结果显示，患儿ｓＩＬ－２Ｒ水平与正常对照组（２５０．９４±９１．４５ｋＵ／Ｌ）比较，化疗前初诊时急性期组（５２６．３２±２８５．６５ｋＵ／Ｌ）及复发组（５５０．００±４１１．２６ｋＵ／Ｌ）明显增高（Ｐ＜０．０１），完全缓解组（２５０．７１±１１７．３１ｋＵ／Ｌ）则无显著差异（Ｐ＞０．０５）。血清ｓＩＬ－２Ｒ水平的变化与外周血及骨髓白血病细胞数呈正相关。提示ｓＩＬ－２Ｒ水平可作为急性白血病患儿病情监测及疗效判断的指标之一。

The levels of serum soluble interleukin-2 receptor(sIL-2R)were determined in 65 children with acute leukemia using enzyme-linkedimmunosobent assay (ELISA).The results demonstrated that the sIL-2R levels in acute leukemia at diagnosis(mean value 526. 32±285.65ku/L) and in relapse (mean value 550.00±411. 26 kU/L) were significantly higher than those in normal control(mean value 250. 94±91. 45 kU/L) (P<0.01 ).There was no difference about sIL-2R levels between acute leukemia at complete remission (meam value 250. 71±117. 31kU/L)and in normal control (P>0.05). The levels were correlated with the number of leukemic blasts in peripheral blood and in bone marrow. These results suggest that the detection of serum sIL-2R levels is a useful clinical indicator in monitoring different phases of acute leukemia and the effectiveness of treatment.