摘要
用放射自显影和免疫印迹方法探讨了热应激蛋白（ＨＳＰ）在多种细胞株中的诱导、定位和分布。结果发现，与正常情况相比，各种细胞在热休克后均可诱导合成大量ＨＳＰ８２．３、ＨＳＰ６９．５和ＨＳＰ２６．８，其合成高峰为受热后４～６ｈ。在正常情况下，ＨＳＰ６９．５和ＨＳＰ２６．８主要位于胞浆，在热休克后（４３～４５℃）迅速向核内迁移，在恢复期，它们又回到胞浆。
The present article investigated the induction, localization and redistribution of heat stress proteins in many kinds of cell lines with autoradiography and western blot. The results showed that heat shock induced the syntheses of HSP 82.3, HSP 69. 5 and HSP 26. 8 in various kinds of cell lines and the peak of the syntheses arrived at the fourth to sixth hour after exposure to heat. HSP 69.5 and HSP 26.8 are mainly located in the cytoplasm under normal conditions, moved from the cytoplasm to the nuleus after heat shock(43～45℃),and returned to the cytoplasm during the recovery. These results indicated that HSP play an important role in the process.
出处
《同济医科大学学报》
CSCD
北大核心
1995年第6期419-421,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金
关键词
热应激蛋白
热休克
heat shock
heat stress proteins
redistribution
