猪肺动脉缺氧内皮细胞条件培养液和DDPH对肺动脉平滑肌细胞增殖的影响

Effects of Hypoxic Porcine Endothelial Cell-conditioned Medium and DDPH on Proliferation of Pulmonary Arterial Smooth Muscle Cells

用３Ｈ－胸腺嘧啶核苷和３Ｈ－尿嘧啶核苷掺入法及流式细胞术，观察猪肺动脉缺氧内皮细胞条件培养液（ＨＥＣＣＭ）和１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙氨基）丙烷盐酸盐（ＤＤＰＨ）对猪肺动脉平滑肌细胞（ＰＡＳＭＣｓ）ＤＮＡ、蛋白质合成及细胞周期的影响。结果显示：ＨＥＣ－ＣＭ可促进ＰＡＳＭＣｓ的ＤＮＡ合成，促进ＰＡＳＭＣｓ由静止期（Ｇ０／Ｇ１）进入ＤＮＡ合成期（Ｓ期）及有丝分裂期（Ｇ２＋Ｍ期），但对其蛋白质合成无明显影响。ＤＤＰＨ对ＨＥＣＣＭ引起ＰＡＳＭＣｓ的ＤＮＡ合成增高的抑制率为５９．７１％，对Ｇ０／Ｇ１期细胞进入Ｓ期增高的抑制率为２２．５８％，此外，ＤＤＰＨ对ＰＡＳＭＣｓ蛋白质的合成也有抑制作用，抑制率为２４．９３％。

The effects of the medium conditioned by hypoxically cultured endothelial cells(HECCM)and 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethy -lamino)propane hydrochloride(DDPH)on DNA, protein synthesis and cell cycle of the porcine pulmonary arterial smooth muscle cells(PASMCs )were studied using ￣3H-thymidine and ￣3H-uridine incorporation and flow cytometry. The results showed that HECCM stimulated DNA synthesis of PASMCs and promoted PASMCs from static phase(G_0/G_1)to DNA synthetic phase (S)and mitotic phase(G_2+M), but it had no obvious effect on protein synthesis of PASMCs. The rate of inhibition of DDPH on increased DNA synthesis of PASMCs induced by HECCM was 59.71%.The rate of inhibition on increased PASMCs from G_0/G_1 phase to S phase was 22. 58%, In addition, DDPH could decrease the protein synthesis of PASMCs(24.93%).