时间分辨免疫荧光法分析CEA的条件研究

STJDIES ON EXPERIMENTAL CONDITIONS OF DETECTING CEA BY TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY

报道了应用时间分辨免疫荧光氏分析血清中ＣＥＡ的条件研究。用我们研制的Ｎ￣１－（对－异硫氰基苄基）－二乙三胺－Ｎ￣１，Ｎ￣２，Ｎ￣３，Ｎ￣３－四乙酸铕为螯合剂，用Ｅｕ标记了抗ＣＥＡ单克隆抗体（ＭｃＡｂ）Ｃ＿（１７）。在免疫分析程序中，对包被的ＭｃＡｂ的种类、浓度和封闭时间、第一和第二次反应的温育时间、标记Ｃ＿（１７）的浓度、分析缓冲液等主要实验条件，进行了实验最佳化选择。分析方法的灵敏度为１ｎｇ／ｍｌ。

The experimental conditions of detecting carcinoembryonic antigen (CEA)by means of time-resolved immunofluorometric assay are studied.A chelate-Eu￣(3+)-N￣1-(p-isothiocyanatobenzyl)-di-ethylenetriamine-N￣1,N￣2,N￣3,N￣3-tetraacetic acid synthesized is used to label mouse monoclonal antibody(McAb)C_(17)against CEA. In the assay,the CEA standard is first reacted with the coated McAb.Afterwashing ,the labeled C_(17) is added and the second reaction is followed.The important experimental con-ditions such as the coated McAbs a nd their concentration,block ing time ,incubation time for the firstand second reaction,the concentration of labeled C_(17) and the assay buffer solution,etc. are selectedand optimized in the assay procedures. the sensitivity of the assay is 1 ng/ml.