光合细菌产氢条件的研究

STUDIES ON THE CONDITIONS OF HYDROGEN-PHOTOPRODUCTION BY PHOTOSYNTHETIC BACTERIA

从被有机物污染的土壤及水域中分离得到13株属于红螺菌科的光合细菌,对其在苹果酸、乳酸、葡萄糖、乙酸、丙酸及丁酸中的光致产氢现象进行了研究。最高产氢量为39.8ml·20ml菌液^(-1)·48h^(-1),产氢活性为7.8ml·g生物量^(-1)·h^(-1)”。底物对不同菌株的产氢量与产氢活性均具有影响,pH对产氢过程也有明显的作用,大于6000lx的光强度对提高产氢活性已无明显作用。固定化细胞在静态培养条件下也能提高产氢能力,但延长产氢时间的作用不明显。

13 pure cultures of Rhodospirillaceae have been isolated from soil and water samples polluted by organic wasteproducts. The H2-photoproduction (HP) and its activity (AHP) were studied and found their maximum values are 39.8 ml · 20ml bacterial suspension-1 · 48h-1 and 7. 8ml · g cell dry wt. -1 ·h-1 respectively. Substrate exerts a grest influence on HP and AHP of all 13 strains, pH value also affects the evolving process evidently, and light intensity higher than 6000lx cannot further increases the AHP. Under static culture conditions cell immobilization is able to raise the H2-photoproduction, but gives no evident effect on its duration.