一种新的农用抗生素——宁南霉素

A NEW AGRICULFURAL ANTIBIOTIC——NINGNANMYCIN

诺尔斯链霉菌西昌变种(Streptomyces nourtei var. xichangensis)发酵液,经提取分离和冷冻干燥,高效液相色谱纯化,得化合物C_(16)H_(25)N_7O_8,经UV、IR、NMR、MS光谱分析和对水解产物的研究,证明为胞嘧啶核苷肽型抗生素,与谷氏菌素的结构差别仅在于谷氏菌素中的丝氨酸为D型,而新分离的宁南霉素则是L型丝氨酸,两者的生物活性差异显著。

A new antibiotic was isolated from fermentation broth of Streptomyces noursei var. xichangensis by cationic ion exchanger, macroporous weak cationic exchanger and neutral aluminium oxide column chromatographies and purified by high performance liquid chromatography. Its molecular weight was determined by FDMS as 444(M +1). The formula C16H25N7O8 was based on the results of the elemental analysis. The structure was elucidated by means of UV-, IR-, 1H-NMR-, 13C-NMR-3 1HH-COSY-, 13CH-COSY,13CH-COLOC-, and MS spectra to give B. However it does not identified with gougerotin or qinfengmycin, compared with their Rf value on paper chromatogramm and biological activities. It shows intensive effect to Xan-thomonas campestvis pv., Erysiphe graminis, Erysiphe cichoracearum. From its hy-drolyt it was isolated and identified as 1(4-amino-4-deoxy β-D-glucoronyl) cysto-sine, L-serine, sarcasine and NH.C1. Therefore, the new agricultural antibiotic Ning-nanmycin belongs to a cystosine nuclecide antibiotic structural same as gougerotion but isomer to gougerotin, the latter is different by D-serine only.