摘要
通过对球形红杆菌(Rhodobacter sphaeroides)生长和积累聚卢-羟基链烷酸(PHA)条件的研究,确定采用两段培养法提高PHA的产量。第一阶段提供适合菌体生长的条件:以葡萄糖作碳源,尿素为氮源,光照微好氧培养。第二阶段则提供使菌体积累PHA的条件:补加乙酸钠厌氧光照培养。经两段培养后菌体PHA含量可占细胞干重的45％,PHA产量每升发酵液可达1.7g。
Through investigating the culture conditions of Rhodobacter sphaeroi-des, a two-stage culture technique was developed for the accumulation of poly (3-hydroxyalkanoates) (PHA) by this microorganism. In the first stage, to provide suitable conditions for the microbial growth glucose and urea were supplied as carbon and nitrogen sources, respectively, keeping a microaerobic light culture. In the second stage, the culture was supplemented with sodium acetate to create an anaerobic light culture condition for the accumulation of PHA. By this technique, the PHA content accumulated in the microbial cells mounted up to 45% of cellular dry weight, and a high yield of 1.7g PHA/L fermenting liquor was achieved.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第6期438-441,共4页
Acta Microbiologica Sinica
关键词
球形红杆菌
聚β羟基链烷酸
积累
Rhodobacter sphaeroides, Poly (3-hydroxyalkanoates), Two-stage cu- lture technique
