混合单克隆抗体ELISA直接法和夹心法检测包虫病人循环抗原

The Comparison of Direct－ELISA and Sandwich－ELISA for Detecting Circulating Antigens in Patients with Hydatid Disease

本文对混合单克隆抗体ＥＬＩＳＡ直接法和ＥＬＩＳＡ夹心法检测包虫病人循环抗原的效果进行了比较研究。结果表明，ＥＬＩＳＡ夹心法对细粒棘球蚴囊液纯化抗原的最低检出量（１．２ｎｇ／ｍｌ）较直接法（１０ｎｇ／ｍｌ）低，但检测包虫病人循环抗原时，ＥＬＩＳＡ直接法的阳性检出率为５６．３９％（２７／４８），ＥＬＩＳＡ夹心法为６０．４％（２９／４８），二者无显著性差异（Ｐ＞０．０５）。两法同时检测１４份猪囊尾蚴病人血清和４４份正常人血清，均未出现假阳性反应。

The circulating antigen in patients with hydatid disease was detected by Direct－ELISA and Sandwich－ELISA using a mixture of monoclonal antibodies. The results showed that the minimal detectable level of Echinococcus granulosus cyctic fluid antigen (EgCFAg) by Sandwich－ELISA(1. 2ng/ml)was lower than that by Direct－ELISA(10ng/ml) ,but for detecting circulating antigen (CAg) in patients sera,the CAg positive rate of the Sandwich－ELISA was 60. 4% (29/48)and 56.3% (27/48)in the Direct－ELISA, it was no significant difference (p>0. 05). 14 samples of patients with human cycticercosis and 44 samples of normal sera were tested by Direct－ELISA and Sandwich－ELISA at the same time. The results showed that there were not false positive reaction in these two methods.