致肾盂肾炎大肠杆菌粘附特性的研究

STUDIES ON THE ADHERENCE PROPERTIES OF UROPATHOGENIC ESCHERICHIA COLI

本文对临床肾盂肾炎病人尿标本中分离的大肠杆菌１３２和１３６的粘附特性进行了系统的研究。受试菌的Ｐ血型阳性红细胞血凝试验阳性，能够与人的尿道上皮细胞粘附。利用致肾盂肾炎大肠杆菌Ｐ菌毛粘附基因群抗血清进行免疫学检测，两株菌的全菌ＥＬＩＳＡ结果阳性，免疫电镜证实该抗血清能与受试菌株的菌毛特异性结合。提取临床分离株的菌毛蛋白进行免疫印迹测定，仅有一条蛋白带显色，其分子量为１６．６ｋｄ。致肾盂肾炎大肠杆菌的粘附特性是区别于其他大肠杆菌的重要特征，上述结果表明本文报告的两株大肠杆菌为致肾盂肾炎大肠杆菌。

E. coli strains 132 and 136 isolated from urine of patients with pyelonephritis were sty-died on their adherence properties. The tested strains were found to agglutinate with the humanerythrocytes of P blood group and to attach to human urinary tract epithelial cells.The immuno-logical examination of the two strains was carried out with the antisera against the P pilus adhe-sive cluster of uropathogenic E. coli. The resuIts of their whole-bacterium ELISA showed to bepositive, and the antisera could be specifically combined with the P pili of E. coli 132 and 136 inthe immunogold electron microscopy. The crude pilus extracts of the two strains were preparedrespectively and tested by SDS-pAGEand western blotting. It was shown that the Mr of the Ppilus proteins from the tvvo clinical strains being 16.6Kd.Uropathogenic E. coli differs greatlyfrom other E. cofi strains in their adherence properties. So the above results revealed that E. coli132 and 136 were characterized as uropathogenic E. coli strains.