产β－甘露聚糖酶地衣芽孢杆菌的分离筛选及发酵条件

THE STUDY ON SELECTION AND FERMENTATION CONDITION OF PRODUCING β-MANNANASE FORM BACILLUS LICHENIFORMIS

从上样中分离筛选出产β－甘露聚糖酶的地衣芽孢杆菌，经紫外线诱变处理后（１５Ｗ，３０ｃｍ照射４０ｓ）获得高酶活力ＮＫ－２７菌株，在以魔芋粉、豆饼粉为碳、氮源添加无机盐的发酵培养基中，β－甘露聚糖酶活力达１１０．４９ｕ／ｍｌ。初始ＰＨ值、装液量、培养温度和培养时间对产酶有一定影响。

A Bacillus lichenlformis of producing β-mannanase were isolated from soil.After treatmentwith U V(15W,30cm,time 40s)a mutant NK-27 producing 110.49u/ml of β-mannase activity wasobtained from 430 strains。The medium for fermentation consisted of Konjak powder,soybean,yeastextract and salt.The primary pH Values, culture temperature and culture time were effective toproduction of β-mannanase。