中性蛋白酶发酵工艺优化研究

STUDY ON THE OPTIMIZATION OF NEUTRAL PROTEINASE FERMENTATION PROCESS

采用正交试验，对枯草芽孢杆菌ＨＤ４０１中性蛋白酶深层发酵工艺进行了优化，结果表明：较优发酵培养基为玉米粉５％、豆饼粉３％、麸皮４％、Ｎａ＿２ＨＰＯ＿４０．４％、ＫＨ＿２ＰＯ＿４０．０３％；在发酵中期流加蚕蛹水解液５％、植酸钙０．２％、吐温－８００．１％，对发酵后期有较大的调控作用。采用优化工艺，在２Ｌ发酵罐中进行验证试验，酶活达１３２００ｕ／ｍｌ，比原工艺提高了１６４％。

By study on the fermentation conditons of the Bacillus Subtilis HD-401 which Producedneutral proteinase, the experimental results showed that the most suitable culture medium wascontained 5%maize flour,3%been cable,4%wheat bron,0.4%NaHPO_4 and 0.03%KH_2PO_4.Thecontrol of this process was concentrated on adding silkworm chrysalis hydrolyzat,plant acid calciumand Tween-80 at 20hours by orthogonal experiment, Enzyme activity could raise to 13200 u/ml byadding 0.5%silkworm chrysalis hydroiyzat,0.2%plant acid caicium and 0.1%Tween-80 in 2Lfernentor。