海藻酸铝固定化酵母生产高浓度酒精的研究

STUDY ON HIGH CONCENTRATION OF ETHANOL FERMENTATION OF IMMOBILIZED GROWING YEAST

以海藻酸铝凝胶代替海藻酸钙，可使固定化酵母使用寿命显著延长，其海藻酸铝凝胶耐磷酸盐能力比海藻酸钙凝胶提高六倍以上。用海藻酸铝固定化增殖酵母进行分批发酵酒精，成熟醪中酒精含量由３．５％－９．０％（Ｖ／Ｖ）提高到１１．０％（Ｖ／Ｖ）左右。在１．１Ｌ的两个多层生物反应器中，装入海藻酸铝固定化增殖酵母，采用逐步提高糖浓度方法，进行连续发酵，成熟醪接收器中酒精浓度平均为１０．３％（Ｖ／Ｖ），总糖利用率为９２．４％。

The useful life time of immobilized yeast was prolonged greatly whenalginate calcium replied by alginate aluminium. The ability of enduring phosphate ofalginate aluminium gel was improved over six times than that of alginate calcium gel.Theconcentration of ethanol in final broth is increased from 8.5-9.0%(V/V)to about 11.0%(V/V).The final concentration ofethanol of continuous fermentation in two 1.1L multi-storey bioreactor filled with immobilized growing yeast in AL-Alg gel, by the way ofimproving the concentration of sugar step by step,could be 10.3%(V/V)at average,andthe utilization ratio of total sugar is 92.4%。