妥布霉素产生菌诱变育种的研究

STUDY ON BREEDING BY INDUCED-MUTATION OF TOBRAMYCIN PRODUCING STRAIN

本实验以妥布霉素产生菌黑暗链霉菌（Ｓ．ｔｅｎｅｂｒａｒｉｕｓ）ＡＴＣＣ１７９２０为出发菌株，经紫外线处理，获得一株产量较高且稳定的菌株ＵＶ－５９，其抗生素效价比原株提高９２．３％对ＵＶ＿５９菌株进行高温处理，得到一株Ｔ－５４１菌株，所产抗生素只有两个组分，比原出发株减少一个组份，不再含有出发株产生的氨甲酸卡那霉素；再对Ｔ－５４１菌株进行原生质体制备，分别用紫外线和紫外线加氯化锂以及亚硝基胍诱变处理原生质体，获得四株高产菌株，效价比原株提高１１５～１５０％，且稳定。此外还研究了变异菌株的形态与产量的关系。

A starting strain, the tobranlycin producing Streptomyces tenebrariusATCC 17920 was treated with UV,the high-yield stable strain UV-59 was obtained, and the production increased 92.3% over its parent strain. Then UV-59 was treated with high temperature,and T-541 was obtained.The antibiotics produced by T-541 only consisted of two kinds of components,and had on aminoformyl kanamycin B produced by the parent strain. Theprotoplast made from T-541 was treated with UV, UV-LiCl and NTG, four high-yield strains were obtained,the increase being 115-150% overthe yield by its. parent strain. Furthermore, they are all stable.The relation between the production and the morphology of the variant strainwas studied.