膳食维生素B_6对硒的生物利用率影响的研究　Ⅰ．对补硒大鼠组织硒含量的影响

Effect of dietary vitamin B_6 on selenium bioavailability Ⅰ.Effect of dietary vitamin B_6 on selenium concentrations in the tissues of rats sodium selenite of DL-selenomethionine

研究膳食维生素Ｂ６（ＶＢ６）对大鼠组织中硒水平的影响。喂饲４周龄断乳雄性大鼠缺ＶＢ６缺硒的酪蛋白蔗糖基础膳食，２周后按体重把动物分成１０组。实验是２×２×２析因设计，２个水平ＶＢ６（每克膳食含０和２．５０μｇ盐酸吡哆醇），２种形式硒（亚硒酸钠和ＤＬ－硒蛋氨酸），２个硒水平（每公斤膳食含０．５和５．０ｍｇ硒），加上２个缺硒组（缺ＶＢ６和补ＶＢ６）。实验期为４周。结果在饲硒蛋氨酸的缺ＶＢ６组，血浆硒水平高于对应的补ＶＢ６组，但是缺ＶＢ６各组的红细胞中硒含量均显著低于补ＶＢ６各组。缺ＶＢ６时，饲亚硒酸钠动物的骨骼肌和心肌中硒水平均显著低于补ＶＢ６的动物；饲硒蛋氨酸大鼠的骨骼肌、心肌、脾、肝和肾脏中硒含量均显著高于补ＶＢ６组。提示ＶＢ６参与了硒在体内的转运以及硒蛋氨酸在肝脏中的代谢过程。

Rats were fed a vitamin B6-Se-deficient basal diet for 2 weeks, and then divided into 10 groups of 5 or 6 rats and fed respective diets for 4 weeks.The experimental design was a 2 × 2 × 2 factorial with two levels of vitamin B6,two forms of Se,and two levels of Se plus two extra groups(vitamin B6 supplemented and deficient without Se).Vitamin B6 levels were 0 and 2.50kg/g of diet;selenium forms were Na2SeO3 or DL-selenomethionine;selenium levels were 0.5 and 5.0mg/kg of diet.Vitamin B6-deficient diets resulted in higher Se level in plasma of rats fed selenomethionine than vitamin B6-supplemented diets.However,selenium content in erythroctes was significantly elevated in vitamin B6 supplemented rats compared with vitamin B6-deficient rats.Selenium levels in muscle and heart were significantly lower in vitamin B6-deficient groups fed Na2SO3 than in vitamin B6-supplemented groups,vitamin B6 deficient rats fed selenomethionine had higherselenium levels in muscle,heart,spleen,liver and kidney than vitamin B6-supplemented rats.These results suggest that vitamin B6 is involved in the transportation of selenium in body and in the metabolism ofselenomethionine in liver.