摘要
米酵菌酸是椰毒假单胞菌及其酵米菌亚种的毒性代谢产物之一，是引起酵米面和变质银耳等多种食品中毒致死的主要病因。作者成功地制备了米酵菌酸牛血清白蛋白结合物（ＢＡＢＳＡ）、卵清蛋白结合物（ＢＡ－ＥＡ），并在国内首次获得了抗ＢＡ半抗原的多克隆抗体，血清效价可达１：１２×１０４和１：８×１０４。建立了检测ＢＡ的直接和间接竞争ＥＬＩＳＡ法。为进一步研制抗ＢＡ的单克隆抗体、探寻特异性的免疫治疗手段提供了有价值的科学基础。
Bongkrekic acid is one of the toxic metabolites produced by pseudomonas cocovenenans,and is the major pathogen causing the poisoning of fermented corn flour,deteriorated tremella and various other foods.Two kinds of hapten albumin conjugates,bongkrekic acid- bovine serum albumin and bongkrekic acid-egg albumin conjugates,were successfully prepared and the polyclonal antibody against bongkrekic acid were obtained firstly in domestic and abroad.The titers of the antisera were 1:120000 and 1:80000,respectively.Both direct and indirect competive Enzyme-Linked Immunosorbent Assays for the detection of BA were established.
出处
《卫生研究》
CAS
CSCD
北大核心
1995年第2期93-95,共3页
Journal of Hygiene Research
基金
卫生部科研基金
