低亲和力抗tpA单克隆抗体在tpA纯化中的应用

APPLICATION OF LOW AFFINITY MONOCLONAL ANTIBODY AGAINST tpA IN PURIFICATION OF tpA

用低亲和力组织型纤溶酶原激活剂（ｔｐＡ）Ａ链单克在抗体制备了免疫亲和层析柱，纯化５０ＯｍｌＢｏｗｅｓ细胞无血清培液中的ｔｐＡ，回收率达７５％，经ＳＤＳ一ＰＡＧＥ电泳证实纯化的洗脱液蛋白的分子量为６７ｋｄ，仅呈一条带，用发色底物法（Ｓ２３９０）测定表明洗脱液蛋白具有ｔｐＡ活性。

mmunoaffinity chromatographic column Was prepared with low affinity monoclonal anti-body against A chain of tissue一type plasminogen activator. tpA was purified from 500ml BOwescell culture without serum. The rate of recovery was up to 75%. Through SDS一PAGE elec- trophoresis it was proven that the molecular weight of the eluted protein purifed was 67 Kd andonly one band was presented. The tpA activity of the eluted protein was identified with chro- mogenic substrate(S2390).