硒酸酯多糖抗石英细胞毒性的研究

RESEARCH OF ANTAGONISM OF KAPPASELENOCARRAGEENAN TO CYTOTOXITY OF QUARTZ

本文采用家兔肺泡巨噬细胞（ＡＭ）体外培养法，以ＡＲ－ＣＭ阳离子测定系统和荧光试剂Ｆｕｒａ－２．研究了石英细胞毒性与肺泡巨噬细胞胞浆游离Ｃａ（２＋）浓度（［Ｃａ（２＋）］ｉ）的关系．并以细胞存活率、乳酸脱氢酸（ＬＤＨ）活性和脂质过氧化物（ＬＰＯ）表征了由石英引起的ＡＭ毒性及硒酸酯多糖对其毒性的拮抗效应，初步讨论了硒酸酯多糖拮抗石英细胞毒性的可能机理．

In this article, the relation between the eytotoxicity of quartz and the change of cytoplasmic free Ca(2+) of alveolar macrophage was studied by means of AR-CM cation measurement system and fluorescence solvent Fura-2. Both the cytotoxicity of quartz and the antagonized effects of Kappa-selenocarrageenan were characterized by the survival percent of cells, the activities of LDH and LPO as well. In addition, the mechanism of Kappa-selenocarragcenan's suppressing cytotoxicity of quartz was also disscussed.