模拟污水中氮、磷对水稻幼苗过氧化氢酶和乙醇酸氧化酶的影响

EFFECTS OF MIMIC WASTEWATER CONTAINING N, P ON ACTIVITIES OF CATALASE AND GLYCOLATE OXIDASE IN THE SEEDLINGS OF RICE

以水稻为材料，研究了不同浓度的模拟污水中Ｎ、Ｐ对幼苗过氧化氢酶（Ｃａｔａｌａｓｅ，ＣＡＴ，ＥＣ１．１１．１．６）、乙醇酸氧化酶（Ｇｌｅｃｏｌａｔｅｏｘｉｄａｓｅ，ＧＯ，ＥＣ１．３．３．１）、过氧化物酶（Ｐｅｒｏｘｉｄａｓｅ，ＰＯＤ，ＥＣ１．１１．１．７）和叶绿素含量的影响．结果表明：低浓度Ｎ、Ｐ（分别；在０～４．２８ｍｍｏｌ·Ｌ－１、０～０．２ｍｍｏｌ·Ｌ－１范围）可诱导ＣＡＴ活性增大，而后下降，Ｎ、Ｐ浓度分别超过８．５６ｍｍｏｌ·Ｌ－１和０．４ｍｍｏｌ·Ｌ－１后，活性又升高；ＰＯＤ的活性变化与ＣＡＴ变化很相似．Ｎ、Ｐ浓度分别在０～６．４２ｍｍｏｌ·Ｌ－１、０～０．３ｍｍｏｌ·Ｌ－１范围，ＧＯ活性随Ｎ、Ｐ浓度增高而活性下降，超过６．４２ｍｍｏｌ·Ｌ－１、０．３ｍｍｏｌ·Ｌ－１后，则随Ｎ、Ｐ浓度增大而增大．这可能是高浓度Ｎ、Ｐ可提高水稻的光呼吸的结果．另外，污水中Ｎ、Ｐ抑制叶绿素的合成，使叶绿素的含量降低；低浓度污水Ｎ、Ｐ促进根生长，高浓度Ｎ。

In this paper,effects of N,P of mimic wastewater on activities of Catalase,Glycolate oxidase,Peroxidase in the seedlings of rice were studied under various concentrations.The results showed:lower concentrations(NT<4.28 mmol. L-1,PT<O. 2 mmol. L-1)increased the activities of CAT,then declined. While NT>8. 56 mmol. L-1 ,PT>0. 4 mmol·L-1, the activites went up again with increase of concentrations of N, P of mimic wastewater.The activities of POD showed similiar results. When NT<6.42 mmol. L-1 and PT<0. 3mmol. L-1,GO activities declined while N,P concentrations increased,inversely,when NT>6. 42 mmol. L-1 and PT>0. 3 mmol. L-1,GO activities increased in direct ratio with N,P concentrations. This showed that higher N,P concentrations increased the intensity of photorespiration in rice,and then affected the activities of CAT and POD. In addition,N,P of the mimic wastewater inhibited synthesis of chlorophyll, and roots growth were hampered by higher concentrations one.