用原位杂交技术检测二价染色体上黄鳝rRNA基因多态性的研究

STUDIES ON POLYMORPHISMS OF rRNA GENES ON PACHYTENE BIVALENTS OF RICE-FIELD EELS BY IN SITU HYBRIDIZATION

用地戈辛标记的原位杂交技术研究黄鳝二价体上ｒＲＮＡ基因的多态性．共检测到：数目和位置多态；杂交信号形态多态；ｒＲＮＡ基因的联合现象；７号二价体上可移动的ｒＲＮＡ基因位点；ｒＲＮＡ基因的串联重复；ｒＲＮＡ基因杂交信号周围的微信号密集等６种多态现象．还对本研究结果进行了详细地讨论．

The polymorphisms of rRNA genes on pachytene bivalents of rice - field eels were studied by using in situ hybridization labelled with digoxigenin. Results of detecting were as follows:polymorphisms of numbers and locations,size polymorphisms of hybridization signals,associated phenomenon of rRNA genes,removable rRNA gene location on No. 7bivalents, tandem repeat of rRNA genes and density of microsignals surrounded by hybridization signals of rRNA genes. And the results studied above were also discussed in details.