HPGMR农垦58s光敏感期叶片中阳离子过氧化物酶的变化

THE CHANGE OF THE CATIONIC PEROXIDASE IN THE LEAVES AT THE FERTILE TRANSFORMATION PERIOD IN HPGMR NONGKEN 58s

以农垦５８ｓ水稻（ＯｒｙｚａｓａｔｉｖａＬ．ｓｕｂｓｐｊａｐｏｎｉｃａ）为材料，用分光光度法和聚丙烯酰胺凝胶电泳，分析了５８ｓ在１０ｈ／ｄ短日照（５８ｓ－ＳＤ）和自然不育长日照（１４．１２ｈ／ｄ左右，５８ｓ－ＮＤ）条件下，育性转换光敏感期，阳离子过氧化物酶活性和同工酶的变化。结果表明，在育性转换期阳离子过氧化物酶活性随幼穗发育时期的推进而升高，在ＮＤ条件下，酶活性升高更快。在所测定的２个时期中，由聚丙烯酰胺凝胶电泳图谱可以看出，５８ｓ－ＳＤ和５８ｓ－ＮＤ均有５条阳离子酶带。在第一次枝梗分化期（Ⅱ），５８ｓ－ＮＤ的第４、５条酶带比５８ｓ－ＳＤ相应酶带的活性强，而到花粉母细胞减数分裂期（Ⅵ），５８ｓ－ＳＤ的第４条酶带活性相对增高，但５８ｓ－ＮＤ的酶活性仍然强于５８ｓ－ＳＤ。这暗示了５８ｓ光敏感期内，光照条件对阳离子过氧化物酶活性的影响可能主要反映在第４、５条酶带上。

The cationic peroxidase change was studied at fertile transformation stage of Nongken 58s rice (Oryza sativa L.subsp japonica)at the different condition of the illumination.The result shows that cationic peroxidase activity increases to be depended on progress of young panicle development.It has a high value in the condition of nature long illumination(i.e.58s-ND)than that in short day(i.e, 58s-SD).Five bands of cationic peroxidase isozymes were separated and can be seen in gel electrophoresis zymogram at the two stages(differentiation of primary brache primordum (Ⅱ)and meiotic division(Ⅳ)of pollen mother cell).At the stage of differentiation of primary brach primordun activity of the fourth and fifth band in condition of ND is stronger than in SD.But at meiotic stage of mother pollen cell,activity of fourth band in SD was increased. However activity of the fourth and fifth band of the isozymes in ND was still stronger than that in SD.It may reflect that the effect of illumination condition on the cationic peroxidase isozymes in photoperiod sensitive stage of fertile transformation in Nongken 58s is mainly related to the fourth and fifth band of zymograms.