摘要
以农垦58s水稻(OryzasativaL.subspjaponica)为材料,用分光光度法和聚丙烯酰胺凝胶电泳,分析了58s在10h/d短日照(58s-SD)和自然不育长日照(14.12h/d左右,58s-ND)条件下,育性转换光敏感期,阳离子过氧化物酶活性和同工酶的变化。结果表明,在育性转换期阳离子过氧化物酶活性随幼穗发育时期的推进而升高,在ND条件下,酶活性升高更快。在所测定的2个时期中,由聚丙烯酰胺凝胶电泳图谱可以看出,58s-SD和58s-ND均有5条阳离子酶带。在第一次枝梗分化期(Ⅱ),58s-ND的第4、5条酶带比58s-SD相应酶带的活性强,而到花粉母细胞减数分裂期(Ⅵ),58s-SD的第4条酶带活性相对增高,但58s-ND的酶活性仍然强于58s-SD。这暗示了58s光敏感期内,光照条件对阳离子过氧化物酶活性的影响可能主要反映在第4、5条酶带上。
The cationic peroxidase change was studied at fertile transformation stage of Nongken 58s rice (Oryza sativa L.subsp japonica)at the different condition of the illumination.The result shows that cationic peroxidase activity increases to be depended on progress of young panicle development.It has a high value in the condition of nature long illumination(i.e.58s-ND)than that in short day(i.e, 58s-SD).Five bands of cationic peroxidase isozymes were separated and can be seen in gel electrophoresis zymogram at the two stages(differentiation of primary brache primordum (Ⅱ)and meiotic division(Ⅳ)of pollen mother cell).At the stage of differentiation of primary brach primordun activity of the fourth and fifth band in condition of ND is stronger than in SD.But at meiotic stage of mother pollen cell,activity of fourth band in SD was increased. However activity of the fourth and fifth band of the isozymes in ND was still stronger than that in SD.It may reflect that the effect of illumination condition on the cationic peroxidase isozymes in photoperiod sensitive stage of fertile transformation in Nongken 58s is mainly related to the fourth and fifth band of zymograms.
出处
《武汉植物学研究》
CSCD
1995年第2期157-162,共6页
Journal of Wuhan Botanical Research
基金
国家自然科学基金
关键词
光敏核不育
水稻
阳离子
过氧化物酶
Photoperiod sensitive genic male-sterile rice,Cationic peroxidase,Isozyme