甘蓝型油菜子油分的积累与某些生理变化关系的研究

STUDIES ON THE RELATIONSHIP BETWEEN OIL CONTENT AND THE CHANGE OF BIOLOGICAL METABOLISM IN BRASSICA NAPUS L. SEED

油菜种子发育过程中，其内部的生理代谢过程发生了规律性的变化。伴随着种子的发育进程，６－磷酸葡萄糖脱氢酶、异柠檬酸裂解酶、异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均有不同程度的增强。在油分旺盛合成期，６－磷酸葡萄糖脱氢酶和异柠檬酸裂解酶的活性均达到了最大值，而此时，异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均增加较慢；在种子的不同发育时期，高含油量品系的６－磷酸葡萄糖脱氢酶和异柠檬酸裂解酶的活性均高于低含油量的品系，而其异柠檬酸脱氢酶和琥珀酸脱氢酶的活性均低于低含油量的品系。高、低含油量不同的品系之间的正反交Ｆ１代其种子在不同的发育时期，上述各种酶的活性均介于双亲之间。

Changes of biological metabolism were happened during the seed development of rapeseed. There were gradually increased in activities of glucose-6-phosphate dehydrogenase, isocitrate lyase, isocitrate dehydrogenase and succinate dehydrogenase. The activities of glucose-6-phosphate dehydrogenase and isocitrate lyase increased to maximum ,however,the activities of isocitrate dehydrogenase and succinate dehydrogenase increased slowly when oil synthesized rapidly. During whole developing stages of rapeseed, the glucose-6-phosphate dehydrogenase and isocitrate lyase in different strains with high oil content were more active than with low ones; on the contrary, isocitrate dehydrogenase and succinate dehydrogenase in strains with low oil content were more active than with high ones. The activities of four enzymes in hybrids were expressed between the range of parents.