纤维素水解产物对木霉A10纤维素酶的抑制作用

Product Inhibition of Hydrolysates to Cellulase of Trichoderma viride A10

应用双层平板牛津杯法对绿色木霉Ａ１０产生的纤维素酶的水解产物抑制作用进行了初步研究，得出了透明圈直径和抑制浓度关系的数学模型，实验结果表明甘油、葡萄糖和纤维二糖的最低抑制浓度分别为３．３５，２．５７和１．８１ｍｍｏｌ／Ｌ其抑制系数分别为２．６４，４．００和６．７０。

As hydrolysis proceeds,the enzyme reaction of extracellular cellulase from Trichodermaviride A10,like other cellulase ,slows due to product inhibition. By use of cylinder plate,product inhi-bition is tested for its effect on cellulase in the cellulotic fungus A10.The experimental results showthat the lowest inhibitor concentration of glycerine,glucose and cellobiose are ca.3. 35,2. 57 and1. 81 m mol/L. And their inhibiting coefficients are ca.2. 64,4. 00 and 6. 70,respectively.