基因工程菌株EscherichiacoliC600（pRLK14）的流加培养

Fed-batch Culture of Escherichia coli C600 Harboring Recombinant Plasmid pRLK14

在１Ｌ搅拌发酵罐中对含有重组质粒ｐＲＬＫ１４的大肠杆菌进行了培养。结果表明，采用二段培养法，于３４℃增殖细菌，在对数增殖后期升温到４２℃进行诱导，可以高效表达基因产品半乳糖激酶。诱导期，醋酸浓度增长较快，模拟实验表明，控制醋酸浓度可进一步提高半乳糖缴酶产率。

An Escherichia coli strain harboring recombinant plasmid pRLK14 was cultured in 1 Litrefermentor.The results showed that genetic product galactokinase could be efficiently produced bytwo-step culture method,in which the cell was grown at 34℃ then shifed to 42℃ during the late loga-rithmic growth phase. Acetic acid concentration was increasing rapidly in the inducible stage。So theproductivity of galactokinase can be raised by controling acetic acid concentration at lower level。