二茂铁衍生物电催化氧化NADH及催化速率常数的测定

Studyon Electrocatalytic Oxidation of NADH by Ferrocene DeriVatives and Determination of Catalytic Rate Constant at Microdisk Electr ode

用微盘电极研究了二茂铁衍生物的电化学行为及电催化氧化二氢烟酰胺腺嘌吟二核苷酸（ＮＡＤＨ）。用微电极测定了二茂铁衍生物（磺酸基二茂铁，乙酰基二茂铁，羧酸基二茂铁，α－羟乙基二茂铁，α，α＇－二经乙基二茂铁）的扩散系数（分别为３．４×１０￣（－６），６．９×１０￣（－６），１．７×１０￣（－６）．６．２×１０￣（－７），１．５×１０￣（－６）ｃｍ￣２／ｓ）及乙酞基二茂铁电催化氧化ＮＡＤＨ的催化速率常数（４．６８×１０￣３（ｍｏｌ／Ｌ）￣（－１））。探讨了温度、ｐＨ及β－环糊精的络合效应对催化反应的影响。

The electrocatalytic oxidation of NADH by ferrocene derivatives and the influence ofcomplexation with β-cyclodextin(β-CD)were investigated at microdlsk electrode in a buffer solution。According to the cyclic voItammetric behavior of the ferrocene derivatives on microdisk electrode,theelectron-transfer rate constant from NADH to the ferricium species was determined.The diffusioncoefficient of ferrocene derivatives were determined with microdisk electrode,the effect of temperatureand pH on elecrtrocatalytic oxidation of NADH were assessed。