花粉浓度测定方法的研究

Studies on Methods of Determination of Pollen Concentration

用４种方法对大小不同的花粉的浓度进行测定。结果表明，各种测定方法的变异系数的大小为：Ｄａｖｉｓ法＜直接计数法＜血球计数器法Ⅱ＜血球计数器法Ⅰ；不同大小花粉测定的变异系数为：小花粉（１０～２５μｍ）＜中等的花粉（２５～５０μｍ）＜大花粉（５０～１００μｍ）＜很大的花粉（１００～２００μｍ）。所研究的４种方法完全适用于小型花粉的浓度测定，体积在中等以上的花粉，测定浓度应选用Ｄａｖｉｓ法和直接计数法。

Four methds were used in determination of pollen concentration.The results show that the order of coefficients of variation was as follows:Davis method<direct count method<methdl Ⅱof blood counter<method Ⅰ of blood counter,and small pallen (10￣25μm)<middle pollen (25￣50μm)<large pollen (50￣100μm)<very large pollen(100￣200μm).The four metnods were suitable for small pollen.Davis method and direct count method were applicable when the size of pollen is larger than middle.