小鼠腔前卵泡卵母细胞的采集和体外成熟

Isolation and in Vitro Maturation of Mouse Oocytes from Preantral Follicles

酶消化配合机械分离，采集１０日龄小鼠腔前卵泡中的卵母细胞一颗粒细胞复合作（ＯＧＣｓ），以自制的胶原膜为底物，在 ＭＥＭ培养液中培养 １０ ｄ．卵母细胞－颗粒细胞复合体由 １１０． ５±１８． ６ μｍ增长到 １８４．４±６０． ６ μｍ，卵母细胞由 ５６． ２５±２． ０ μｍ增长到 ８２． ０±１０． ５μｍ．３６．７％的ＯＧＣ．具有５层以上颗粒细胞，其中９．１％放出第一极体。

The method of enzymatic digestion cooperating with mechanical isolation for 15 minutes was used to collect oocyte-granulosa cell complexes (OGC,) from preantral follocles of 10-day-old mice. The OGC. were cultured in MEM medium on selfmade collagen membranes for 10 days. The diameter of OGC. increased from 110. 5 ± 18. 6 μm to 184. 416±. 6 μm, the diameter of oocytes increased from 56. 25±2. 0 μm to 82. 0± 10. 5 μm. 36. 7% of oocytes gets 5 or more layers of granulosa cells, 9. 1% of which were extruded a polar body.