EDTA对水稻γ射线慢照射效应的影响

INFLUENCE OF EDTA ON CHRONIC γ-RAY RADIATION EFFECTS IN RICE

γ射线慢照射水稻分蘖一籽粒腊熟中期植株,在剂量率小于0.1089GY/h,总剂量虽已达到236GY,但对当代(M_1)的株高、结实率仍无明显辐射损伤.EDTA溶液浸种后再照射植株以及照射过程中叶面喷EKTA溶液,均有增加辐射损伤趋势,但作用不显著.各种处理的M_2代均无叶绿素突变产生.但在M_3代,除单一ED-TA溶液浸种的无叶绿素突变出现外,其他复合处理和单一γ射线照射的均有叶绿素突变产生,其中EDTA溶液浸种后再照射的植株突变频率显著高于其他处理.慢照射过程中叶面喷EDTA溶液增加突变频率的作用不显著.文章讨论了剂量与辐射损伤的关系.

Rice plants were treated with either chronic 7-ray or EDTA and chronic γ-ray complex. The influence of EDTA on chronic radiation effects of rice plants was studied. The results showed that radiation injuries were not increased obviously with EDTA soaking seeds before irradiation or spraying EDTA solution upon plants during chronic radiation. It was also observed that no chlorophyll mutations were found in all treatments in the M2 generation, but in the M3 progeny chlorophyll mutaions were ascertained in each treatment except non-irradiated control. The frequency of chlorphyll mutation in case of EDTA solution soaking seeds before irradiation was significantly higher than other treatments. Total frequency of mutation in M3 progenies with EDTA solution soaking seeds before irradiating was increased compared with other treatments except for control. The frequency of mutations was not increased with spraying EDTA solution upon plants during the peri-odof chronically radiating plants. The relationship between radiation injury and the rate of exposure was discussed.