玉米花粉的冷藏研究Ⅴ．玉米花粉离体培养中几种培养基的比较

Study on refrigeration in maize pollenⅤ Comparison of culture mediums in maize pollen culture

选择适宜的培养基是花粉离休培养的重要环节．１０种培养基培养结果表明，以Ｃａ（ＮＯ＿３）＿２０．０３％，ＭｓＳＯ＿４０．０２％，ＫＮＯ＿３０．０１％，硼酸０．０１％，蔗糖１５％，琼脂１％的配方更为适宜，可使玉米花粉的萌发率达８５％以上．

The selection of culture medium is a key stop in pollen culture. 10 kind of culture mediums in malze pollen culture are compared and the result shows,The available culture medium consists ofCa(NO_3)_2 0.03%,MgSO_4 0.02%,KNO_3 0.01%,H_3BO_3 0.01%,Sucrose 15% and agar 1%.85percent of germination rate of maize pollen have been obtained.