荻不同外植体离体培养研究

STUDY ON THE CULTURE OF DIFFERENT EXPLANTS OF MISCANTHUS SACCHARIFLORUS(MAXIM.)BENTH ET HOOK IN VITRO

用荻无菌苗的下胚轴、芽鞘、幼根、幼叶、无菌小苗和幼穗为外植体，以ＭＳ＋２，４－Ｄ２ｍｇ／Ｌ＋６－ＢＡ０．１ｍｇ／Ｌ为愈伤组织诱导培养基；ＭＳ＋６－ＢＡ１．５ｍｇ／Ｌ＋水解乳蛋白４００ｍｇ／Ｌ为分化培养基；１／２ＭＳ＋ＮＡＡ０．２５ｍｇ／Ｌ＋ＩＡＡ０．２５ｍｇ／Ｌ为生根培养基进行组织培养。结果表明：幼叶离体培养未产生愈伤组织；幼根能产生大量松散的愈伤组织，尚未分化出苗；下胚轴和芽鞘的愈伤组织能分化出苗；无菌小苗仅从根、胚轴、芽鞘处诱导出愈伤组织，只有胚轴、芽鞘愈伤组织能分化苗；幼穗培养产生大量愈伤组织，而且在ＭＳ＋２，４－Ｄ ２ｍｇ／Ｌ＋６－ＢＡ０．１ｍｇ／Ｌ培养基上继代培养，从紫色愈伤组织表面形成淡黄色致密的胚性愈伤组织，分化绿苗的频率可达７５％。荻幼穗离体培养是理想的外植体。

Explants were hypocotyls,bud sheathes,young roots ,young leaves ,aseptic seedlings, youngspikelets of matural plantlets of Miscanthus sacchariflorus(Maxim)Benth.et Hook.Inducedmedium for callus was MS medium with 2 mg/L2,4-D and 0.1mg/L 6BA. Differentiationmedium for bud was MS medium with 1.5mg/L 6BA and 400 mg/L lactalbumin hydrolysate.Induced medium for root was1/2 MS medium loith 0.25mg/L NAA and 0.25mg/L IAA.Theresults indicated:callus did not formed in young leaf culture.A lot of loose calli formed formyoung root in vitro,but there were no buds formed in culture.Some buds differentiated fromthese calli from hypocotyls and bud sheathS Calli were in duced only from root,plumular axis andbud sheaths of little aseptic seedlings, but shoots formed only from these calli from plumular axisand bud sheath.Calli were induced largely in young spikelet culture,when they were subculturedon MS medium with 2 mg/L2,4 D and 0. 1mg/L 6BA,embryonic calli which were light yel-low in colour and close in texture formed on the brown callus surface.The differentiation rato ofgreen shoots can reach 75%.The young spikelets were the best explants in the culture ofMiscanthus sacchariflorus (Maxim) Benth et Hook in vitro.