两个发根农杆菌菌株对马铃薯的高效遗传转化

EFFICIENT TRANSFORMATION OF POTATO(SOLALUM TUBEROSUML.)USING TWO STRAINS OF AGROBACTERIUM RHIZOGENES

报道了发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）两个菌株Ａ４和ＬＢＡ９４０２Ｂｉｎ１９）对马铃薯（ＳｏｌａｎｕｍｔｕｂｅｒｏｓｕｍＬ．）茎段和叶切片的遗传转化。两个菌株的根诱导频率明显不同，由此得到的毛状根无性系在侧根数和根长度等形态特征上呈现一定的差异，Ａ４的转化频率较高，为２０％—２８％，ＬＢＡ９４０２Ｂｉｎ１９转化频率虽然只有８％—１８％，但仍接近前人报道的结果。这些根无性系可用于研究植株再生和ＲｉＴ—ＤＮＡ对马铃薯块茎发生的影响。

ntroduction Agrobacterium rhizogenes incites hairy root formation in wounded tissues of many dicot plantspecies by transferring specific T DNA into plant genome and this usually involves aux genesand rol genes in the T DNA of Ri plasmid.This technique of genetic transformation has beenapplied to many crop species like those belonging Brassica(He et al.1990 and 1994, Tepfer1984).Potato(Solanum tuberosum L.)is one of the most important food crops in the world.In re-cent years,there were a few of reports about the transformation of different genotypes of potatousing Ti plasmids(Ooms et al.1983 ,Sheerman and Bevan 1988,Visser et al. 1989a) and Ri plasmids(Ooms et al.1985,Hanisch ten Cate et al.1988,de Vries Uijtewaal et al.1988,On the leave from Department of Botany,University of Poona,Pune 411007,India.Acknowledgement: N.P.Malpathak gratefully acknowledges the financial support from UNESCO Biotechnology ActionCouncil for this work.Visser et al.1989b).However,little is known about the effects of Ri T DNA genes on tuberformation although it is known that auxin synthesis genes and rol genes in Ri DNA could regu-late tuber formation.The present work was a part of our research project to stanardize a transformation procedurefor the study of regulatory effects of endogenous hormones on potato tuber initiation and develop-ment.We used two strains of A.rhizogenes (A4 and LBA9402Bin19) to infect stem and leaf ex-plants.Transformation frequency of plant explants and root morphology and opine production ofresultant hairy roots were observed.