植物生长调节剂和蔗糖对抗黑胫病香果树丛芽分化的影响

THE EFFECT OF PLANT GROWTH REGULATORS AND SUGAR ON BUDS DIFFERENTIATION IN THE BLACK LEG DISEASE RESISTANT EMMENOPTERYS HENRYI

将抗黑胫病香果树愈伤组织在ＢＡ、ＮＡＡ、蔗糖３因素条件下作Ｌ＿９（３￣４）正交组织培养试验．结果表明，ＢＡ、ＢＡ与ＮＡＡ的交互作用对抗病香果树愈伤组织丛芽分化有极显著影响．ＢＡ／ＮＮＡ（Ｘ）与芽分化（Ｙ）的关系为Ｙ＝１．３６５１Ｘ￣（０．１２８６），ＢＡ／ＮＡＡ（Ｘ）和ＢＡ＋ＮＡＡ（Ｙ）与芽分化（Ｚ）的关系为Ｚ＝１．１０３３＋０．０９９９（Ｘ＋Ｙ）－０．００２７（Ｘ＋Ｙ）￣２，ＢＡ／ＮＡＡ（Ｘ）和蔗糖浓度（Ｙ）与芽分化（Ｚ）的关系为Ｚ＝１．０７４０＋０．０７９３（Ｘ＋Ｙ）－０．００１７（Ｘ＋Ｙ）￣２．在本该试验范围内，丛芽分化的最适培养基为ＭＳ＋ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ＋蔗糖３％．

Using the callus of black Leg disease resistant Emmenopterys Henryi on MS medium containing threefactors BA,NAA and sugar to do L_9(3￣4)orthogonal tissue culture experiment is successful.The resultsshow that:BA and the reciprocal effect of BA and NAA have conspicuous influence on bud differentia-tion in the black leg disease resistant Emmenopterys Henryi, and describe quantitatively the relation be-tween the ratio of BA/NAA,the total plant growth regulators, sugar and bud differentiation. The rela-tion of bud differentiation (Z)with BA/NAA(X)and sugar (Y) is Z=1.0740+0.0793(X+Y)-0.0017(X+Y)￣2,r￣2=0.8668.The relation of bud differentiation(Z)with BA/NAA(X)andBA+NAA(Y)is Z=1.1033+0.0999(X+Y)-0.0027(X+Y)￣2,r￣2=0.9759.In this experiment,the bet medium of bud differentiation is MS+BA 2.0mg/L+NAA0.1 mg/L+sugar 3%.