沙门氏菌LPS相关抗体的快速检测

A Rapiderodiagnosis for Antibodies Against Salmonella LPS

建立两种颗粒——乳胶颗粒及磁性颗粒快速检测沙门氏菌ＬＰＳ相关抗体（ＩｇＭ或ＩｇＧ的方法。乳胶颗粒用ＬＰＳ包被，磁性颗粒用羊抗人ＩｇＭ或ＩｇＧ包被，将两种颗粒及血清标本置于酶标条的微孔中，室温振荡３０ｍｉｎ后置于磁铁上使磁性颗粒沉淀。上清液在４００ｎｍ的酶标仪上读取浊度以判定反应结果。该方法比乳胶凝集试验的灵敏度高。其结果与用ＥＬＩＳＡ方法检测的结果一致，但步骤少，时间短，操作更简便。是一种快速诊断沙门氏菌感染的有效方法。

An efficient assay has been developed for detection antibodies againstSalmonella lipopolysaccharide(LPS).Latex coated with I/PS,polystyrene beads( Dynabeads )coated with goat anti-human μ or γ,and serum is mixed in the well of micro plate and shaken for30 min at room temperature。Then sediment by a magnet, the turbidity of the resuItant latexsuspension is measured spectrophotometrically at wavelength of 400 nm. The assay gives a highersensitivity as compared with latex agglutination. The results are in accordance with ELISA,while easier and can be accomplished by fewer steps.